Journal: American Journal of Physiology - Renal Physiology

Article Title: Loss of circadian gene Bmal1 in the collecting duct lowers blood pressure in male, but not female, mice

doi: 10.1152/ajprenal.00364.2019

Figure Lengend Snippet: Urinary endothelin (ET)-1 excretion during the active phase of the last day of normal-salt diet (A and D), high-salt diet (B and E), or high-salt diet + the ET type B (ETB) receptor antagonist A-192621 (C and F) treatment. Data are presented for the active phase. Student’s t test was used to compare CDBmal1KO vs. flox mice: *P < 0.05.

Article Snippet: Urinary ET-1 concentrations were determined by ELISA (QuantiGlo ET-1 Kit, R&D Systems, Minneapolis, MN).

Techniques:

Journal: American Journal of Physiology - Renal Physiology

Article Title: Flow regulation of collecting duct endothelin-1 production

doi: 10.1152/ajprenal.00530.2010

Figure Lengend Snippet: Urine flow rate (V), Na+ excretion (UNaV), ET-1 excretion (UETV), and mean arterial pressure (MAP) in control or furosemide-infused rats; n = 5 for control and n = 6 for furosemide. *P < 0.05 vs. control at same time point.

Article Snippet: Urinary, cell, and perfusate ET-1 content were determined using a QuantiGlo ET-1 enzyme immunoassay (R&D Systems, Minneapolis, MN).

Techniques:

Journal: American Journal of Physiology - Renal Physiology

Article Title: Flow regulation of collecting duct endothelin-1 production

doi: 10.1152/ajprenal.00530.2010

Figure Lengend Snippet: Dose response (A) and time course (B) of shear stress-induced alterations in ET-1 mRNA content in mpkCCDc14 cell. For the dose response, cells were exposed to varying flow for 2 h, while for the time course, cells were exposed to a shear stress of 2 dyne/cm2 for varying times; n = 6–14 each data point. *P < 0.05 vs. cells not exposed to flow.

Article Snippet: Urinary, cell, and perfusate ET-1 content were determined using a QuantiGlo ET-1 enzyme immunoassay (R&D Systems, Minneapolis, MN).

Techniques: Shear

Journal: American Journal of Physiology - Renal Physiology

Article Title: Flow regulation of collecting duct endothelin-1 production

doi: 10.1152/ajprenal.00530.2010

Figure Lengend Snippet: Effect of inhibition of cyclooxygenase or nitric oxide synthase on flow-stimulated ET-1 mRNA content in mpkCCDc14 cells. Cells were preincubated with vehicle, 1 mM NG-nitro-l-arginine methyl ester (l-NAME), or 1 μM indomethacin for 30 min, followed by exposure to shear stress at 2 dyne/cm2 for 2 h in the presence of the same agents and then determination of ET-1/GAPDH mRNA levels; n = 6–8 each data point. *P < 0.05 vs. cells treated identically with the same agents, but not exposed to flow (control).

Article Snippet: Urinary, cell, and perfusate ET-1 content were determined using a QuantiGlo ET-1 enzyme immunoassay (R&D Systems, Minneapolis, MN).

Techniques: Inhibition, Shear

Journal: American Journal of Physiology - Renal Physiology

Article Title: Flow regulation of collecting duct endothelin-1 production

doi: 10.1152/ajprenal.00530.2010

Figure Lengend Snippet: Effect of modulation of Ca2+ pathways on flow-stimulated ET-1 mRNA content in mpkCCDc14 cells. Cells were preincubated with vehicle, HBSS without Ca2+, 50 μM BAPTA (to chelate intracellular Ca2+), 100 μM nifedipine, or 10 μM W-7 (CaM inhibition) for 30 min, followed by exposure to shear stress at 2 dyne/cm2 for 2 h in the presence of the same agents/media and then determination of ET-1/GAPDH mRNA levels; n = 6–8 each data point. *P < 0.05 vs. cells treated identically with the same agents, but not exposed to control.

Article Snippet: Urinary, cell, and perfusate ET-1 content were determined using a QuantiGlo ET-1 enzyme immunoassay (R&D Systems, Minneapolis, MN).

Techniques: Inhibition, Shear

Journal: American Journal of Physiology - Renal Physiology

Article Title: Flow regulation of collecting duct endothelin-1 production

doi: 10.1152/ajprenal.00530.2010

Figure Lengend Snippet: Effect of modulation of protein kinase C (PKC) and phospholipase C (PLC) on flow-stimulated ET-1 mRNA content in mpkCCDc14 cells. For phorbol 12-myristate 13-acetate (PMA) studies, cells were preincubated with vehicle or 50 ng/ml PMA for 2 h. Otherwise, cells were incubated with vehicle, 100 nM calphostin C (PKC inhibitor), or 2 μM U73122 (PLC inhibitor) for 30 min. After preincubations, cells were exposed to shear stress at 2 dyne/cm2 for 2 h in the presence of the same agents and then determination of ET-1/GAPDH mRNA levels; n = 6–8 each data point. *P < 0.05 vs. cells treated identically with the same agents, but not exposed to flow (control).

Article Snippet: Urinary, cell, and perfusate ET-1 content were determined using a QuantiGlo ET-1 enzyme immunoassay (R&D Systems, Minneapolis, MN).

Techniques: Incubation, Shear

Journal: Function (Oxford, England)

Article Title: Timing of Food Intake Drives the Circadian Rhythm of Blood Pressure

doi: 10.1093/function/zqaa034

Figure Lengend Snippet: Urine volume (A), urinary sodium excretion (UNaV; B), urinary potassium excretion (UKV; C), urine excretion of ET-1 (D), and aldosterone (E) during ad libitum feeding and RF in WT mice. Data are presented as 12-h average of the last 2 days. Repeated measures two-way ANOVA indicated significant Feeding × Time interactions ( P < 0.05; bars indicate significant post hoc comparisons).

Article Snippet: Urinary endothelin-1 (ET-1) concentrations were determined by ELISA (QuantiGlo ET-1 Kit, R&D Systems, Minneapolis, MN).

Techniques:

Journal: Function (Oxford, England)

Article Title: Timing of Food Intake Drives the Circadian Rhythm of Blood Pressure

doi: 10.1093/function/zqaa034

Figure Lengend Snippet: Urine volume (A), urinary sodium excretion (UNaV; B), urinary potassium excretion (UKV; C), urine excretion of ET-1 (D), and aldosterone (E) during ad libitum feeding and RF in Bmal1KO mice. Data are presented as 12-h average of the last 2 days. Repeated measures two-way ANOVA was used.

Article Snippet: Urinary endothelin-1 (ET-1) concentrations were determined by ELISA (QuantiGlo ET-1 Kit, R&D Systems, Minneapolis, MN).

Techniques: